transfection human bmscs  (Procell Inc)

Journal: Scientific Reports

Article Title: Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway

doi: 10.1038/s41598-025-33075-7

Figure Lengend Snippet: Identification of BMSCs and BMSCs-derived Exos. ( A ) Alkaline phosphatase and Alizarin red S were used to determine the multipotential differentiation capabilities of BMSCs; ( B ) Characteristic surface markers of BMSCs evaluated by flow cytometry; ( C ) The morphology of exosome (100 μm) was observed under a TEM; ( D ) Nanoparticle tracing assay of the BMSCs-derived Exos; ( E ) The protein levels of CD9, CD63, CD81, TSG101, and Hsp70 were measured by Western blot. Data are presented as mean ± SD ( n = 3 independent biological replicates).

Article Snippet: Human bone mesenchymal stem cells (BMSCs) obtained from Procell Biotechnology Co. LTD (Wuhan, China) were cultured in commercial BMSCs complete medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO 2 .

Techniques: Derivative Assay, Flow Cytometry, Western Blot

Journal: Scientific Reports

Article Title: Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway

doi: 10.1038/s41598-025-33075-7

Figure Lengend Snippet: BMSCs-derived exosomes promoted osteogenic differentiation in hFOB1.19 cells via activation of the Wnt/β-catenin signaling pathway. ( A ) ALP staining was performed to assess osteogenic differentiation in each group; ( B ) Alizarin red S staining was performed to detect the mineralized area in each group; Relative mRNA and protein levels of RUNX2, OCN, OPN, Osterix, and ALP in each group were analyzed by ( C ) RT-qPCR and ( D ) Western blot; ( E ) Western blot was performed to detect the protein levels of Wnt1, β-catenin, GSK3β, and Cyclin D1 in each group. Data are presented as mean ± SD ( n = 3 independent biological replicates). *** p < 0.001 vs. the BMSCs-PBS group, ### p < 0.001 vs. the BMSCs-Exo group.

Article Snippet: Human bone mesenchymal stem cells (BMSCs) obtained from Procell Biotechnology Co. LTD (Wuhan, China) were cultured in commercial BMSCs complete medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO 2 .

Techniques: Derivative Assay, Activation Assay, Staining, Quantitative RT-PCR, Western Blot

Journal: Scientific Reports

Article Title: Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway

doi: 10.1038/s41598-025-33075-7

Figure Lengend Snippet: Inhibition of BMSCs-derived exosomal miR-509-5p impaired osteogenic differentiation via suppression of the Wnt/β-catenin pathway in hFOB1.19 cells. ( A ) miRNA qPCR array-based detection of differentially expressed miRNAs between the BMSCs-PBS and the BMSCs-derived exosomes group; ( B ) The expression of miR-15b-5p, miR-24-3p, miR-148a-3p, miR-181b-5p, and miR-509-5p in BMSCs-PBS and the BMSCs-derived exosomes group were detected by RT-qPCR; ( C ) RT-qPCR was performed to assess the mRNA level of miR-15b-5p after miR-15b-5p inhibition; ( D ) ALP staining was performed to assess osteogenic differentiation in each group; ( E ) Alizarin red S staining was performed to detect the mineralized area in each group; ( F-G ) Relative mRNA and protein levels of RUNX2, OCN, OPN, Osterix, and ALP in each group were analyzed by RT-qPCR and Western blot; ( H ) Western blot was performed to detect the protein levels of Wnt1, β-catenin, GSK3β, and Cyclin D1 in each group. Data are presented as mean ± SD ( n = 3 independent biological replicates). * p < 0.05, *** p < 0.001 vs. the PBS group, ### p < 0.001 vs. the BMSCs-Exo + NC inhibitor group.

Article Snippet: Human bone mesenchymal stem cells (BMSCs) obtained from Procell Biotechnology Co. LTD (Wuhan, China) were cultured in commercial BMSCs complete medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO 2 .

Techniques: Inhibition, Derivative Assay, Expressing, Quantitative RT-PCR, Staining, Western Blot

Journal: Scientific Reports

Article Title: Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway

doi: 10.1038/s41598-025-33075-7

Figure Lengend Snippet: Overexpression of BMSCs-derived exosomal miR-509-5p promoted osteogenic differentiation via activation of the Wnt/β-catenin pathway in hFOB1.19 cells. ( A ) RT-qPCR was performed to assess the mRNA level of miR-15b-5p after miR-15b-5p overexpression; ( B ) ALP staining was performed to assess osteogenic differentiation in each group; ( C ) Alizarin red S staining was performed to detect the mineralized area in each group; ( D-E ) Relative mRNA and protein levels of RUNX2, OCN, OPN, Osterix, and ALP in each group were analyzed by RT-qPCR and Western blot; ( F) Western blot was performed to detect the protein levels of Wnt1, β-catenin, GSK3β, and Cyclin D1 in each group. Data are presented as mean ± SD ( n = 3 independent biological replicates). ** p < 0.01, *** p < 0.001 vs. the miR-NC or the BMSCs-PBS group, ### p < 0.001 vs. the BMSCs-Exo + miR-NC group.

Article Snippet: Human bone mesenchymal stem cells (BMSCs) obtained from Procell Biotechnology Co. LTD (Wuhan, China) were cultured in commercial BMSCs complete medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C and 5% CO 2 .

Techniques: Over Expression, Derivative Assay, Activation Assay, Quantitative RT-PCR, Staining, Western Blot

Journal: Journal of Materials Science. Materials in Medicine

Article Title: Copper tannic acid coordination nanosheet as a potent in-situ antibiotic sustained-release carrier for chronic osteomyelitis

doi: 10.1007/s10856-025-06979-z

Figure Lengend Snippet: The effect of Van@CuTA on BMSCs and HUVECs. A CCK-8 assay demonstrated no toxicity of Van@CuTA toward BMSCs. B Representative images of alizarin red staining, revealing increased calcium deposition in BMSCs treated with Van@CuTA. C , D Representative images and statistical analysis of HUVECs migration assay demonstrating a significant increase in migrated cells following Van@CuTA treatment. E – G Tube formation assay images and corresponding quantifications of total tube length and node number per view indicated that Van@CuTA significantly promoted HUVECs’ tube formation. (* indicated p < 0.05)

Article Snippet: Primary bone marrow mesenchymal stem cells (BMSCs) and Human Umbilical Vein Endothelial Cells (HUVECs) were obtained from ATCC.

Techniques: CCK-8 Assay, Staining, Migration, Tube Formation Assay